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原装正品|GenMute siRNA Transfection Reagent for Primary Keratinocytes

产品编号: SL100568-KER     查看说明书
产品名称: 原装正品|GenMute siRNA Transfection Reagent for Primary Keratinocytes  .0   订购此产品 
供应商: SignaGen
规格: 1.0 ml
目录价: 1980
库存状态: 询盘
CAS编号:
应用范围: 转染试剂
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原装正品|GenMute siRNA Transfection Reagent for Primary Keratinocytes
Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.
Size & content:
GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
Summary of Optimal Transfection Conditions:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~70%
Optimal siRNA concentration    40 nM
Diluent for siRNA and GenMute™ Reagent   1xGenMute™ Transfection Buffer
Incubation Time to Form GenMute™/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 90%
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

保存条件: 4°C
说明书地址: 点击查看详细
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