|
原装正品|GenMute siRNA Transfection Reagent for Primary Keratinocytes
Description:
GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.
Size & content:
GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
Summary of Optimal Transfection Conditions:
Cell culture medium DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection ~70%
Optimal siRNA concentration 40 nM
Diluent for siRNA and GenMute™ Reagent 1xGenMute™ Transfection Buffer
Incubation Time to Form GenMute™/siRNA Complex ~15 minutes at RT
Maximal Silencing Efficiency 48 hours
Transfection Results:
Reporter Gene Renilla Luciferase
Silencing Efficiency (% ) 90%
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |