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原装正品|GenMute siRNA Transfection Reagent for Primary Macrophages

产品编号: SL100568-PMG     查看说明书
产品名称: 原装正品|GenMute siRNA Transfection Reagent for Primary Macrophages  .0   订购此产品 
供应商: SignaGen
规格: 1.0 ml
目录价: 1980
库存状态: 询盘
CAS编号:
应用范围: 转染试剂
种属来源:
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原装正品|GenMute siRNA Transfection Reagent for Primary Macrophages
Description:
GenMute™ siRNA Transfection Reagent for PMG is pre-optimized for transfecting siRNA to primary macrophages. Macrophages are white blood cells within tissues, produced by the differentiation of monocytes. Monocytes and macrophages are phagocytes, acting in both non-specific defense (innate immunity) as well as to help initiate specific defense mechanisms (adaptive immunity) of vertebrate animals. Their role is to phagocytose (engulf and then digest) cellular debris and pathogens either as stationary or as mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen. They can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), Lysozyme M, MAC-1/MAC-3 and CD68 by flow cytometry or immunohistochemical staining.
Summary of Optimal Transfection Conditions:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~90%
Optimal siRNA concentration    50 nM
Diluent for siRNA and GenMute™ Reagent   1xGenMute™ Transfection Buffer
Incubation Time to Form GenMute™/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 85%
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
.

保存条件: 4°C
说明书地址: 点击查看详细
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