Tri-DAP L-Ala-γ-D-Glu-mDAP (Tri-DAP) comprises the iE-DAP (γ-D-Glu-mDAP) dipeptide and a L-Ala residue. Tri-DAP is present in the peptidoglycan (PGN) of Gram-negative bacilli and certain Gram-positive bacteria. Tri-DAP is recognized by the intracellular sensor NOD1, which induces a signaling cascade leading to NF-κB activation and the production of inflammatory cytokines. Tri-DAP exhibits a ~3-fold higher ability to activate NF-κB than iE-DAP. Tri-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1. Note: Tri-DAP is a mixture of L-Ala-γ-D-Glu-D-mDAP and L- Ala-γ-D-Glu-L-mDAP. Specificity: NOD1 agonist Working Concentration: 100 ng - 10 µg/ml Endotoxin level: <0.125 EU/ml Solubility: 10 mg/ml in water Formula: C15H26N4O8 Molecular weight: 390.39 Synonym: L-alanyl-γ-D-glutamyl-meso-diaminopimelic acid