|
ODN 4084-F
Toll-Like Receptor 9 (TLR9) detects unmethylated CpG dinucleotides in bacterial or viral DNA inducing strong immunostimulatory effects. TLR9 activation can be mimicked by synthetic phosphorothioate-stabilized oligodeoxynucleotides (ODN) containing immune stimulatory “CpG motifs”. Recent studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs.
ODN 4084-F belongs to a new class of inhibitory ODNs [1-2]. It contains an inhibitory DNA motif consisting of two nucleotide triplets, a proximal CCT and a more distal GGG, spaced from each other by four nucleotides. ODN 4084-F is the shortest active inhibitory ODN. ODN 4084-F is linear and a class B (‘broadly-active') inhibitory ODN. ODN 4084-F is potent inhibitor of TLR9-induced B cells and macrophages [3].
Specificity: Class B inhibitory ODN
Working concentration: 100 nM - 10 µM
Solubility: 2 mg/ml in water
ODN 4084-F sequence
5’- cctggatgggaa -3’
Note: Bases are phosphorothioate (nuclease resistant).
Quality control
- The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells. |