5′ppp-dsRNA Control Negative control for 5′ppp-dsRNA 5’ppp-dsRNA Control is a 19 mer blunt-end dsRNA without a 5’triphosphate. 5’ppp-dsRNA Control is negative control for 5’ triphosphate double stranded RNA (5’ ppp-dsRNA) a synthetic ligand for RIG-I. 5’ ppp-dsRNA is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake. Working Concentration: 300 ng- 1 μg/ml Endotoxin level: EndoFit™ (<0.001 EU/µg) Solubility: 0.1 mg/ml in water Formulation: naked 5’ ppp-dsrna control 5’- GCAUGCGACCUCUGUUUGA -3’ (19 mer) 3’- CGUACGCUGGAGACAAACU -5’ (19 mer) The double stranded RNA Control is obtained by hybridization of non-triphosphatase single-stranded 19-mer phosphodiester RNA with its complementary strand (also non-triphosphatase). Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.
