ODN 2216 Biotin Biotin labeled CpG oligonucleotide - Human TLR9 ligand CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules. ODN 2216 Biotin is a A-class CpG ODN specific for human TLR9. ODN 2216 Biotin can be used to evaluate CpG ODN cellular uptake and localization using a biotin detection system and light microscopy. Specificity: Human TLR9 agonist Working concentration: 10 ng -10 μg/ml ODN 2216 sequence 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer) Note: Bases shown in capital letters are phosphodiester, and those in lower case are phosphorothioate (nuclease resistant). Palindrome is underlined.
