ODN 2006

ODN 2006 (ODN 7909)
Class B CpG oligonucleotide - Human TLR9 ligand
CpG ODNs are  synthetic oligonucleotides that contain unmethylated CpG dinucleotides  in particular sequence contexts (CpG motifs). These CpG motifs are  present at a 20-fold greater frequency in bacterial DNA compared to  mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9)  leading to strong immunostimulatory effects.
ODN 2006 (ODN 7909) is a B-class CpG ODN specific for human TLR9.
ODN 2006 is also available on ready-to-use 8-well strips pre-coated at 7 different concentrations and a negative control.
Synonyms: ODN 7909, PF_3512676
Specificity: human TLR9 agonist
Working concentration: 5 µM (10 μg/ml)
Solubility:  5 mg/ml in water
ODN 2006 sequence
5’-tcgtcgttttgtcgttttgtcgtt-3’ (24 mer)
Note: Bases are phosphorothioate (nuclease resistant).
Quality control
- TLR9 activity is tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (endotoxins, peptidoglycans) is controlled using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.