Hygromycin B

Hygromycin B
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus.It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. Hygromycin B is effective on most bacteria, fungi and higher eukaryotes.
Resistance to hygromycin is conferred by the hph gene from E. coli.
Hygromycin B is normally used at a concentration of 50-200 µg/ml in mammalian cells and 100 µg/ml in bacteria.
InvivoGen also provides HygroGold™, an ultra-pure preparation of hygromycin B.
Working concentration:
- 50-500 µg/ml in mammalian cells
- 50-100 µg/ml in bacteria
CAS number: 31282-04-9
Purity: >85%
Molecular weight: 527.52
Formula: C20H37N3O13, HCl