HygroGold™ HygroGold™ is an ultrapure Hygromycin B. HygroGold™ is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome. HygroGold™ is effective on most bacteria, fungi and higher eukaryotes. Resistance to hygromycin is conferred by the hph gene from E. coli. HygroGold™ is normally used at a concentration of 50-200 µg/ml in mammalian cells and 100 µg/ml in bacteria. Working concentration: - 50-500 µg/ml in mammalian cells - 50-100 µg/ml in bacteria CAS number: 31282-04-9 Purity: >98% Molecular weight: 527.52 Formula: C20H37N3O13, HCl