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Con A Sepharose 4B
Ligand: Concanavalin A (Con A)
Average Particle Size: 90 μm
Matrix: 4% Agarose
Particle Size: 45 µm-165 µm
Ligand Concentration: 13 mg Con A/ml drained medium
pH Stability Working Range: 4-9
pH Stability Cleaning-in-Place (CIP): 4-9
Pressure/Flow Specification: Base matrix 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.
Flow Velocity: < 75 cm/h at 25 ˚C, HR 16/10 column, 5 cm bed
height
Storage Conditions: 2 to 8°C, 0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol
Chemical Stability: Stable to all commonly used aqueous buffers
Avoid Using: Chelating agents such as EDTA, 8 M urea or
solutions having a pH below 3 should be
avoided as these conditions results in removal
of manganese from the lectin with loss of
activity as a result. |