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Ni Sepharose High Performance
Average Particle Size: 34 µm
Matrix: Highly cross-linked agarose, 6%
Binding Capacity/ml Chromatography Medium: At least 40 mg histidine-tagged protein/ml medium1)
Metal Ion Capacity: approx. 15 µmol Ni2+/ml medium
pH Stability Working Range: 3-122)
pH Stability Cleaning-in-Place (CIP): 2-143)
Storage Conditions: 4 to 30°C, 20% Ethanol
Chemical Stability: For Ni2+-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min
Avoid Using: Chelating agents such as EDTA, EGTA , citrate
1)Protein binding capacity is protein-to-protein dependent.
2)Ni²⁺-stripped medium.
3)Ni²⁺-stripped medium.
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