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Ni Sepharose 6 Fast Flow
BioProcess Medium: Yes
Average Particle Size: 90 µm
Matrix: 6% cross-linked agarose
Particle Size: 45 µm-165 µm
Binding Capacity/ml Chromatography Medium: Approx. 40 mg histidine-tagged protein
Metal Ion Capacity: ~15 µmol Ni2₊/ml medium
pH Stability Working Range: 3-121)
pH Stability Cleaning-in-Place (CIP): 2-142)
Pressure/Flow Specification: Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Storage Conditions: 4 to 30°C, 20% Ethanol
Chemical Stability: Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3)
1)Ni²⁺-stripped medium.
2)Ni²⁺-stripped medium.
3)Ni²⁺-stripped medium.
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