pIEx/Bac™-5 3C/LIC Vector Kit This product has been discontinued. The pIEx/Bac™-5 3C/LIC vector carries an N-terminal GST•Tag™ coding sequence followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ_GP, and is active at low temperatures. The 3C/LIC cloning site is followed by an optional C-terminal thrombin recognition site and a Strep•Tag® II coding sequence. This 3C/LIC vector is ready for rapid, directional cloning of PCR-amplified DNA, without restriction enzyme digestion or ligation. The pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. 产品信息 Components • 20 µg (40 µl) 0.5 µg/µl pIEx/Bac-5 3C/LIC DNA • 50 µl T4 DNA Polymerase Buffer • 5 µg (10 µl) 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control • 25 U T4 DNA Polymerase, LIC-qualified • 100 µl 100 mM DTT • 40 µl 25 mM dATP • 50 µl 25 mM EDTA • 1.5 ml Nuclease-free Water • 1 pkg NovaBlue GigaSingles Competent Cells • 2 ml SOC Medium • 10 µl Test Plasmid 商店和运输信息 Storage ≤ -70°C Ship Dry Ice Only Harmful