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pIEx/Bac™-4 Ek/LIC Vector Kit
This product has been discontinued.
The pIEx/Bac™-4 Ek/LIC vector has an N-terminal GST-Tag™ coding sequence followed by a recognition site for enterokinase. The Ek/LIC cloning site is followed by an optional C-terminal His•Tag® coding sequence. This Ek/LIC vector is ready for rapid, directional cloning of PCR-amplified DNA, without restriction enzyme digestion or ligation. The pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus.
产品信息
Components
• 20 µg (40 µl) 0.5 µg/µl pIEx/Bac-4 Ek/LIC DNA
• 50 µl T4 DNA Polymerase Buffer
• 5 µg (10 µl) 0.5 µg/µl pIEx/Bac Ek/LIC Positive Expression Control
• 25 U T4 DNA Polymerase, LIC-qualified
• 100 µl 100 mM DTT
• 40 µl 25 mM dATP
• 50 µl 25 mM EDTA
• 1.5 ml Nuclease-free Water
• 1 pkg NovaBlue GigaSingles Competent Cells
• 2 ml SOC Medium
• 10 µl Test Plasmid
商店和运输信息
Storage ≤ -70°C
Ship Dry Ice Only
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