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iFOLD® Protein Refolding System 1
Purify inclusion bodies and determine optimal protein refolding conditions in a 96-well plate
This product has been discontinued.
Structural and functional proteomics requires large amounts of very pure and correctly folded protein. Due to simplicity, low cost, and potential to express large quantities of target protein, E. coli expression systems are frequently used. However, production of foreign proteins in E. coli may result in the formation of inclusion bodies. Although inclusion body formation is typically not desirable, the aggregates are resistant to proteolysis, easily purified, and can be denatured with chaotropes. Unfortunately, defining conditions that promote refolding of the target protein into its native conformation is empirical and difficult.
The iFOLD® Protein Refolding System 1 is designed to determine optimal protein refolding conditions by a systematic evaluation of 92 different buffers covering a range of pH and different salt, cyclodextrin, redox agent, and refolding additive concentrations. The iFOLD system matrix design is based on an extensive literature review of successful refolding experiments and on information contained in the REFOLD database (http://refold.med.monash.edu. au). The system includes inclusion body purification reagents combined with a 96-well plate-based protein refolding matrix. Following cell lysis, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of detergent and buffer washes. The purified inclusion bodies are denatured by addition of TCEP [Tris(2-carboxyethyl)phosphine] and N-Lauroylsarcosine and refolded by rapid dilution into the iFOLD 96-well buffer matrix. Each kit is sufficient for screening up to 96 refolding conditions (92 experimental and four control wells) for a single protein.
产品信息
Features and benefits • All reagents for inclusion body purification and pre-dispensed iFOLD™ 96-well protein refolding buffer matrix
• Comprehensive set of optimized screening conditions for protein refolding
• Refolding conditions based on extensive literature review
• 96-well refolding plate compatible with HT methodology
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Ship Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS |