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BacMagic™ Transfection Kit
The BacMagic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 Transfection Kit contains the BacMagic™-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
产品信息
Components
• 0.5 µg BacMagic DNA
• 50 µl Insect GeneJuice Transfection Reagent
• 2 x 1 ml Sf9 Cells
• 1 L BacVector® Insect Cell Medium
• 2 µg Transfection Control Plasmid
商店和运输信息
Storage Multiple storage requirements
Ship Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS |