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69671 Thrombin, Restriction Grade
Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
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Product information
Molar mass 37,000
Avoid freeze/thaw Yes
Unit definition One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl2, 50 µg fusion protein.
Components • 50 U Thrombin
• 1 ml 10X Thrombin Cleavage Buffer
• 2 ml 1X Thrombin Dilution/Storage Buffer
• 10 µg Cleavage Control Protein
Source Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
RTECS XO8950000
CAS number 9002-04-4
Store and ship information
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |