His.Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods His-Tag融合蛋白可以在变性条件下纯化，以下是基本的路线。包含体用6M盐酸胍或8M的尿素buffer加BME溶解，亲和树脂用同样的buffer平衡，上样后用同样的buffer加10mM的咪唑洗杂。最后洗脱可采用同样的buffer加200mM的咪唑洗脱或用pH4.5的8M尿素洗脱。上样和洗杂的buffer的pH至少要在7以上 。如果洗脱的蛋白还不够纯，可以进一步提高咪唑的浓度