|
ProteoExtract® Subcellular Proteome Extraction Kit
Fast and reproducible extraction of subcellular proteomes from mammalian cells
ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) is designed for fast and reproducible extraction of subcellular proteomes from adherent and suspension-grown mammalian cells. The S-PEK takes advantage of the different solubilities of certain subcellular compartments in the four selected reagents. In the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. Cells or the parts of the cells remain attached to the plate during sequential extraction of subcellular compartments, until the appropriate extraction reagent is used. Thus, the early destruction of the cellular structure by enzymatic or mechanical detachment of cells from the tissue culture plate and any mixing of different subcellular compartments is prevented. For suspension-grown cells, extraction starts with gentle sedimentation and washing of the cells. The stepwise extraction delivers four distinct protein fractions from one sample:
Cytosolic fraction (F1)
Membrane/organelle protein fraction (F2)
Nucleic protein fraction (F3)
Cytoskeletal fraction (F4)
Proteins are obtained in the native state making the S-PEK suitable for many downstream applications such as 1D and 2D gel electrophoresis, immunoblotting, enzyme activity assays, and protein microarrays.
Sample size: 3-5x106 or 25-50 mg tissue.
产品信息
Form 20 Extractions
Avoid freeze/thaw 是的
Sample type Mammalian tissue, cultured mammalian cells
Kit contains Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
Features and benefits
Stepwise extraction resulting in four distinct subcellular proteomes from one sample
Highly reproducible
No ultracentrifugation steps
Fast—needs just 2 hours with 45 minutes hands-on time
Produces proteins suitable for functional studies
商店和运输信息
Storage +2°C to +8°C
Ship Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |