|
Proteasome Isolation Kit, Human
A rapid method for the isolation of biologically active proteasomes using affinity matrix beads comprised of a GST-fusion protein containing an ubiquitin-like domain (UbL) bound to GST-agarose. The proteasome subunit proteins can be identified by loading the beads directly onto an SDS-PAGE gel and immunoblotting with subunit specific antibodies. Alternatively, proteasome bound to beads can be used in proteolytic assays using proteasome substrates.
• Utilize Ubiquitin-like domain (Ubl) bound to GST-agarose
• Rapidly isolate biologically active proteasomes
• Affinity mature beads can be directly loaded onto SDS-PAGE gels for proteasome subunit identification
• Proteasome-bound beads are compatible with proteolytic assays using proteasome substrates
产品信息
Format 1.0 ml bead suspension
Species reactivity canine, human, mouse
Do not freeze 是的
Assay time 3-5 h
Sample type Cell extracts
Kit contains UbL Beads Sufficient to process 12.5 to 25 mg of Lysate, Control Glutathione-Agarose Beads, Control Lysate, and a user protocol.
Declaration Sold under license of U.S. Patent 6,294,363.
商店和运输信息
Storage +2°C to +8°C
Ship Blue Ice Only
Standard Handling |