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High Pure Viral RNA Kit
Low to medium throughput viral RNA isolation.
Application
Use this High Pure kit to isolate viral RNA from mammalian plasma, serum, other body fluids, or cell culture supernatant. The isolated RNA is suitable for RT-PCR.
Benefits
•Isolates viral RNA from a variety of samples
•Removes inhibitors that might interfere with downstream assays, ensuring greater assay specificity, sensitivity and reproducibility
•Prepares RNA samples in only 20 minutes
•Yields a concentrated sample that is suitable for direct assay (no precipitation required)
•Minimizes RNA loss by eliminating time-consuming steps such as precipitation and solvent extraction
Product Description
Sample
Liquid samples used in research (e.g., serum, plasma, urine, or cell culture supernatant): 200 - 600 μl
Background Information
Nucleic acids (NA) bind to the surface of glass fleece in the presence of chaotropic salt. This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. When the desired product is RNA, the binding conditions can be optimized to ensure immobilization of all the RNA.
Contents
1.Binding Buffer, 2 x 25 ml
2.Poly (A), lyoph., 2 mg
3.Inhibitor Removal Buffer, 33 ml
4.Wash Buffer, 2 x 10 ml
5.Elution Buffer, 30 ml
6.High Pure Spin Filter Tubes (containing glass fiber fleece), 2 x 50
7.Collection Tubes, 8 x 50 |