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High Pure Viral Nucleic Acid Kit
Low to medium throughput viral nucleic acid isolation.
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Application
Use this High Pure kit to isolate viral nucleic acid (DNA and RNA) from mammalian plasma, serum or whole blood. The isolated nucleic acid is suitable for PCR or RT-PCR.
Benefits
•Isolates both viral RNA and DNA, allowing simultaneous detection of both types of virus
•Removes inhibitors that might interfere with downstream assays, ensuring greater assay specificity, sensitivity and reproducibility
•Prepares nucleic acid samples in only 20 minutes
•Yields a concentrated sample that is suitable for direct assay (no precipitation required)
•Procedure is scalable, to accommodate samples with low viral loads
Product Description
Sample
Serum, plasma, whole blood: 200 - 300 μl
Background Information
Nucleic acids (NA) bind to the surface of glass fiber fleece in the presence of chaotropic salt. This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Contents
1.Binding Buffer, 2 x 25 ml
2.Poly (A), lyoph., 2 mg
3.Proteinase K, recombinant, lyoph., 100 mg
4.Inhibitor Removal Buffer, 33 ml
5.Wash Buffer, 2 x 10 ml
6.Elution Buffer, 30 ml
7.High Pure Spin Filter Tubes (containing glass fiber fleece), 2 x 50
8.Collection Tubes, 8 x 50 |