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High Pure PCR Cleanup Micro Kit
Contents
1.Binding Buffer
2.Binding Enhancer
3.Wash Buffer Concentrate
4.Elution Buffer
5.High Pure Micro Filter Tubes
6.Collection Tubes
Application
Use the High Pure PCR Cleanup Micro Kit to efficiently purify products from PCR and other reactions. The kit eliminates contaminants from amplification reactions (e.g., primers, mineral oil, salts) and can also be used to purify nucleic acids from other modification reactions such as restriction endonuclease digests, alkaline phosphatase treatment, and kinase reactions. In addition, the kit can be utilized to purify cDNA, concentrate dilute nucleic acid solutions, and recover DNA from agarose gel slices. The purified DNA can be used directly in subsequent enzymatic reactions such as labeling, sequencing, cloning, and ligation, as well as for PCR analysis.
Benefits
•Conserve resources by using one versatile kit that eliminates the need to use several kits from other suppliers.
•Save time with a simple and rapid protocol that reduces purification time.
•Obtain purified product in a small elution volume (≤ 10 μl) for demanding downstream applications.
•Efficiently remove contaminants and unwanted reaction components.
•Generate contaminant-free DNA for direct use in cloning, ligation, restriction digests, and other reactions.
•Selectively isolate specific DNA fragment sizes by using the kit’s binding enhancer to adjust purification stringency.
Background Information
The amount of DNA recovered is dependent on the amount of DNA applied to the glass fiber fleece, the elution volume, and the length of the amplification/DNA products. When 5 - 25 μg DNA is applied to the kit’s High Pure Micro Filter Tube, approximately 80% of the DNA can be recovered. |