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High Pure miRNA Isolation Kit
Low to medium throughput miRNA isolation.
Contents
Tissue Lysis Buffer (for FFPE sections)
Proteinase K, recombinant (for FFPE sections)
Binding Buffer
Binding Enhancer
Wash Buffer
Elution Buffer
High Pure Filter Tubes
Collection Tubes
Benefits
Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription.
Obtain excellent performance and linearity in RT-PCR.
Save time with a simple, efficient protocol.
Eliminate the need for organic solvent extraction.
Generate stable, highly pure miRNAs.
Background Information
MicroRNAs are natural regulators of gene expression, affecting almost every cellular process (growth and development, cell differentiation, cell death). They occur naturally in diverse animal and plant species, and hundreds have been discovered since the late 1990s. A single miRNA can ramp down expression of multiple genes.
Application
The High Pure miRNA Isolation Kit is designed for the purification and enrichment of small RNAs such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed/paraffin embedded [FFPE] sections) or plant material. It can also be used to purify total RNA or to prepare samples enriched for small RNA <100 nucleotides. The purified RNA is suitable for direct use in downstream applications such as northern blotting, miRNA array hybridization, and relative quantification of miRNA with RT-PCR |