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Genopure Plasmid Maxi Kit
For large-scale (maxi) preparation of plasmid DNA.
Application
Use this kit to prepare "transfection grade" plasmid DNA in large quantities (up to 500 μg plasmid) from bacterial cultures. Plasmid isolated from the “maxi prep” is suitable for most molecular biology applications, including:
•Transfection
•Southern blotting
•Sequencing
•PCR
•Restriction analysis
•Cloning
Benefits
•Requires very little hands-on time (approx. 10 min)
•Saves time, because the overall procedure requires only 75 min
•Works well with all sizes and types of plasmid
•Purifies even large DNA constructs, since the crude lysate may be filtered to avoid plasmid shearing
•Can process several samples in parallel
Contents
1.Suspension Buffer, 150 ml
2.RNase A, 15 mg
3.Lysis Buffer, 150 ml
4.Neutralization Buffer, 150 ml
5.Equilibration Buffer, 70 ml
6.Wash Buffer, 375 ml
7.Elution Buffer, 200 ml
8.NucleoBond AX 500 Columns, 10
9.Folded Filters (240 mm diameter), 10
10.Sealing Rings, 5 |