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GC-RICH PCR System
Optimized system for amplification of difficult targets up to 5 kb by the polymerase chain reaction (PCR).
Contents
1.GC-RICH Enzyme Mix , in storage buffer
2.GC-RICH Reaction Buffer, 5x, with 7.5 mM MgCl2 and DMSO
3.GC-RICH Resolution Solution, 5 M
4.MgCl2 Stock Solution, 25 mM
5.PCR grade water
Application
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance - especially from problematic templates.
•PCR
•Amplification of difficult templates up to 5 kb
Benefits
•Amplify fragments up to 5 kb.
The reagent enables the amplification of a variety of DNA and cDNA fragments.
•Work with difficult DNA templates.
The optimally designed system can amplify difficult templates, including GC-rich targets, repetitive sequences, as well as uniform amplification of a mixture of nucleic acids with varying GC content.
•Benefit from PCR-Grade Water.
The GC-RICH PCR System conveniently provides a GC-RICH Resolution Solution and buffers, with and without Mg, Mg-solution, and PCR-Grade Water.
Quality
The GC-RICH PCR System is function-tested, amplifying a 284 bp fragment of ApoE gene from human genomic DNA, according to the GC-RICH PCR System protocols. |