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FuGENE HD转染试剂
全世界30%的转染用户使用的FuGENE®，其优势在于：
• Off-target效应低 – 转染试剂将外源核酸片段导入细胞的同时，也将影响内源基因表达水平，从而对细胞的代谢和状态造成不利影响，或影响实验数据的客观性，这称为off-target效应。FuGENE®只是LipofectAMINE 2000的16%，试验表明，转染MCF-7 cells (ATCC® HTB-22™)时，采用micro-array检查发现，使用LipofectAMINE 2000转染导致细胞内源1741个基因表达发生变化，而使用FuGENE® HD只有282个内源基因表达发生变化。[发表于Biochemica 4, 22-25 (2006)，请登www.roche-applied-science.com/publications/biochemica.htm阅读电子版]
• 细胞毒性低 – 这是下游实验顺利进行的保证
FuGENE® HD转染试剂
继堪称转染试剂精粹的FuGENE® 6之后，罗氏推出全新的FuGENE® HD，全面提升转染实验结果，为您实验助一臂之力。
• 高效转染目前已有的转染试剂难转染的细胞株，例如：MCF-7，RAW 267.7，PC-3，Hela，MA-10，HepG2，SH-SY5Y，A7r5，STO，SCC-61，STSAR-90，SQ20B，T98G，STSAR-90，A375，A549等；
• 同时适用于昆虫细胞，多种癌症细胞株，原代细胞和干细胞转染；
• 更低的细胞毒性，有助获得更多具有活性的功能细胞，避免和减少使用其他转染试剂导致的对细胞应答和细胞基因表达的影响；
• 显著提升目的基因在常用的细胞株中的蛋白表达水平，通过调整转染试剂的用量，就可轻松优化蛋白表达；
• 更安全可靠的组份，不含任何动物来源成份，经0.1um过滤灭菌，100%血清中依然有活性；
• 稳定的试剂，室温状态下依然稳定；简便的操作步骤，快速实验方案同时适用多种细胞，满足大通量的实验操作需要。
ROCHE相关产品资料
FuGENE HD Transfection Reagent
FuGENE® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.
Application
FuGENE® HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. It is designed to transfect a wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents (e.g ., MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98).
Note: For the most up-to-date list of cell types that have been successfully transfected with FuGENE® HD Transfection Reagent, visit our Transfection Special Interest Site at www.powerful-transfection.com and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.
For life science research only.
Product Description
Composition: unique and proprietary blend of many components, in 80% ethanol.
Storage: stable at +2°C to +8°C.
Stability: 2 years, starting from date of manufacture.
Contents
Solution in 80% ethanol, filter-sterilized (0.1 µm filter), packaged in transparent glass vials.
Quality
Lot-specific analysis:
Each lot of FuGENE®HD Transfection Reagent is carefully checked, using ISO DIN 9001-certified procedures, to assure product is performing according to specifications and is consistent from lot to lot.
Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE® HD and 0.1 µg DNA or 0.25 µl FuGENE® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.
Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay which is an indirect measure of cytotoxicity. The remain |