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FuGENE HD Transfection Reagent
Multicomponent formulation for the transfection of a wide range of eukaryotic cells, for cellular analysis and protein expression. New X-tremeGENE DNA Transfection Reagents available from Roche. Order your free sample at www.x-tremegene.roche.com
Application
FuGENE® HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. It is designed to transfect a wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents (e.g ., MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98).
Note: For the most up-to-date list of cell types that have been successfully transfected with FuGENE® HD Transfection Reagent, visit our Transfection Special Interest Site at www.powerful-transfection.com and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.
For life science research only.
Product Description
Composition: unique and proprietary blend of many components, in 80% ethanol.
Storage: stable at +2°C to +8°C.
Stability: 2 years, starting from date of manufacture.
Contents
Solution in 80% ethanol, filter-sterilized (0.1 µm filter), packaged in transparent glass vials.
Quality
Lot-specific analysis:
Each lot of FuGENE®HD Transfection Reagent is carefully checked, using ISO DIN 9001-certified procedures, to assure product is performing according to specifications and is consistent from lot to lot.
Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE® HD and 0.1 µg DNA or 0.25 µl FuGENE® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.
Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.
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