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FastStart PCR Master
Application
FastStart PCR Master is a ready-to-use, 2x-concentrated hot start master mix. It contains all the reagents (except PCR primers and template) required for running standard PCR and two-step RT-PCR on thermal block cycler instruments.
Hot start PCR
High-throughput PCR
Benefits
Maximize convenience.
All you need to provide is primers, template, and water – the double-concentrated master mix contains everything else you need.
Simplify a variety of PCR applications.
The convenient master mix can be used to amplify fragments in routine, high-throughput PCR, or direct colony PCR.
Improve reliability, and reduce risk of contamination.
Fewer pipetting steps are necessary, thereby limiting sources of error and contamination.
Set up reactions with robotic pipetting stations.
The heat-activated polymerase-based mix is stable for 24 hours at room temperature.
Reduce setup time.
FastStart PCR Master can be stored at +2 to +8°C – ready for immediate use – for up to one month.
Background Information
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. The FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at 95°C).
Contents
FastStart PCR Master, 2x-concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, and Nucleotides (dATP, dCTP, dGTP, dTTP).
Quality
Function test: Each lot of the FastStart PCR Master is function tested in PCR. A 365 bp fragment of the tissue-type plasminogen activator (t-PA) gene and a 1.1 kb fragment of the collagen gene are amplified using human genomic DNA and specific primers. Additionally, each lot is tested for the absence of RNases, DNases, and nicking activities. |