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Expand High Fidelity PCR System, dNTPack
Expand High Fidelity enzyme blend for accurate amplification of genomic DNA targets up to 5 kb by polymerase chain reaction (PCR), with ready-to-use PCR Nucleotide Mix.
Contents
1.Expand High Fidelity Enzyme Mix
2.Expand High Fidelity Buffer, 10x conc. with 15 mM MgCl2
3.Expand High Fidelity Buffer, 10x conc. without MgCl2
4.MgCl2 Stock Solution, 25 mM
5.PCR Grade Nucleotide Mix
Application
The Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length.
•PCR
•RT-PCR
Benefits
The Expand High Fidelity PCR System ensures improved results over Taq Polymerase when comparing yield, fidelity, and flexibility.
•Detect amplification products
that were previously undetectable and avoid false negatives.
•Achieve successful PCR results
from small quantities of template DNA.
•Achieve higher sensitivity
under a wider variety of conditions compared to Taq DNA Polymerase.
•Ensure optimal performance by using PCR-Grade Nucleotides.
Our high-purity nucleotides improve experimental consistency and sensitivity.
Product Description
The enzyme dNTPack comprises the Expand High Fidelity PCR System and a ready-to-use solution of PCR-Grade Nucleotides.
For a standard 50 µl PCR, we recommend using 2.6 U of the enzyme blend. |