Expand High Fidelity PCR System Expand High Fidelity enzyme blend for extremly accurate amplification of genomic DNA targets up to 5 kb by polymerase chain reaction (PCR).Contents1.Expand High Fidelity Enzyme Mix 2.Expand High Fidelity Buffer, 10x conc. with MgCl2 3.Expand High Fidelity Buffer, 10x conc. without MgCl2 4.MgCl2 Stock Solution, 25 mM ApplicationThe Expand High Fidelity PCR System is an enzyme blend containing Taq DNA Polymerase and a polymerase with proofreading activity. Expand High Fidelity PCR System delivers superior results with two-fold higher yield and three-fold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length. •PCR•RT-PCR BenefitsThe Expand High Fidelity PCR System ensures improved results over Taq Polymerase when comparing yield, fidelity, and flexibility.•Detect amplification products that were previously undetectable and avoid false negatives. •Achieve successful PCR results from small quantities of template DNA.•Achieve higher sensitivityunder a wider variety of conditions compared to Taq DNA Polymerase. QualityEach lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1 kb amplified product) and the tPA gene (4.8 kb amplified product).