DIG DNA Labeling Mix Product Description Labeling efficiency: With 1 µg DNA per assay, approx. 10% of the nucleotides are incorporated into about 250 ng of newly synthesized labeled DNA within 1 hour and approx. 30% of the nucleotides into about 750 ng after 20 hours. Contents 10x solution with: 1 mM dATP, dCTP, dGTP (each), 0.65 mM dTTP, 0.35 mM DIG-11-dUTP, alkali-labile. Quality Function-tested in the DIG DNA Labeling Kit and in the DIG Nucleic Acid Detection Kit. Application Random-primed labeling of DNA with DIG-11-dUTP. The labeled probes can be used for Southern, northern, and dot blots, colony and plaque lifts, or in situ hybridization
|