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Application
The Anti-Protein C Affinity Matrix provides a simple and effective method for the immunoprecipitation or column-format purification of protein C-tagged proteins from crude protein mixtures. It is also useful for the rapid immunoaffinity purification of tagged proteins under non-denaturing elution conditions using calcium-chelating reagents (e.g., EDTA), or alternatively with a specific Protein C-tagged peptide.
Anti-Protein C Affinity Matrix is suitable for:
Immunoprecipitation of Protein C-tagged proteins from mammalian, bacterial, and yeast cell extracts
Affinity column purification of Protein C-tagged proteins from crude protein extracts
Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
Western blotting using the Anti-Protein C antibody
Silver staining (or similar protein stain)
Product Description
Specificity: The antibody specifically binds with the 12-amino acid sequence EDQVDPRLIDGK, derived from the human plasma protein Protein C. The Protein C epitope does not contain the Ca2+-binding site. The monoclonal Anti-Protein C antibody (clone HPC4) contains the Ca2+-binding site. In the presence of Ca2+, the antibody binds with high affinity and specificity to this sequence in native human Protein C or in proteins tagged with this epitope. This efficient binding within recombinant fusion proteins, derived from mammalian, bacterial, or yeast expression vectors, occurs regardless of the site of incorporation of the epitope tag (N-terminal, C-terminal, or internal).
Clone: HPC4
Subtype: IgG1κ
Contents
The antibody is covalently coupled to agarose beads and supplied as a 2 ml slurry containing 1 ml beads and 1 ml buffer; 4 mg of antibody is reacted per ml of beads in the coupling reaction. A plastic column with top and bottom caps is included.
11815024001 1 ml settled resin volume
Anti-Protein C Affinity Matrix from mouse IgG1κ | 名称 | 产地 | 货号 | 规格 | 价格 | | Anti-Protein C Affinity Matrix from mouse IgG1κ | ROCHE | 11815024001 | 1ml | 14512 | |