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Product Description
Polyclonal serum
IgG class: rabbit Ig
No. of tests: variable
Species specificity: primates and rodents
Specificity: Poly(ADP-ribose)polymerase (PARP) is a zinc-dependent eukaryotic DNA-binding protein that specifically recognizes DNA-strand breaks that are produced by various genotoxic agents. Recently, it has been reported that PARP serves as a substrate for apoptosis-specific proteases from the ICE family. The protease responsible for the cleavage of PARP was termed apopain, which is derived from its proenzyme Yama/CPP32.
Additionally, CPP32/Mch2, TX, and Nedd-2 were described as PARP-cleaving ICE-like proteases. It has been found that the 113-kD PARP is cleaved during apoptosis into 89-kD and 24-kD fragments, which could serve as an early specific marker of apoptosis. In immunoprecipitation and western-blot experiments, the antibody recognizes full length or fragmented PARP (primates and rodents).
Working concentration: 0.5 µl/ml (western blot)
Figure 1: Detection of PARP cleavage fragments following apoptosis induction of MCF-7 cells. Extracts from treated and untreated cells were analyzed on western blot, followed by indirect immunodetection with Anti-PARP. Results: Anti- PARP recognizes intact (116 kD) and cleaved (85 kD) PARP.
Lane 1: Untreated control cells.
Lane 2: Cells treated with 100 ng/ml doxorubicin for 24 hours.
Lane 3: Cells treated with 1 mg/ml methotrexate for 24 hours.
Lane 4: Cells treated with 1 mg/ml cytarabin for 24 hours.
Application
Western blot, Immunochemistry, Immunoprecipitation | 产品货号 | 名称 | 包装 | 价格 | | 11835238001 | anti-parp | 100UL（50次） | 4562 | |