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Benefits
Safer, since the kit does not use radioisotopes.
Accurate, since results generated with this assay strongly correlate to those obtained with the [3 H]-thymidine method. (See Figure 2 below.)
Sensitive. This assay and the [3 H]-thymidine assay are equally sensitive. (See Figure 2 below.)
Fast, since results can be read with a multi-well ELISA reader, allowing simultaneous processsing of a large number of samples
Easy, since the assay uses a standard cell ELISA protocol.
Economical, since the assay requires no expensive equipment or additional reagents (e.g ., scintillation fluid).
Application
Use this ELISA assay kit to determine the amount of BrdU incorporated into cellular DNA.
Note: The assay is performed in 96-well microplates.
Product Description
Specificity: Antibody specifically binds to 5-bromo-2'-deoxy-uridine that has been incorporated into DNA. It shows no crossreactivity with any endogenous cellular components, such as thymidine or uridine.
Sensitivity: The sensitivity of the BrdU Labeling and Detection Kit III is comparable to that of the traditional [3 H]-thymidine assay. (See Figure 2 below.)
Sample material: Adherent or suspension cells cultured in 96-well microplates.
Background Information
Proliferation in cell populations may be studied by incorporating the radioisotope [3H]-thymidine into cellular DNA. The amount of radioactive thymidine incorporated is determined by scintillation counting.
Alternatively , 5-bromo-2'-deoxy-uridine may be incorporated into cellular DNA. The amount of BrdU incorporated is determined by a standard ELISA protocol, which involves "tagging" the incorporated nucleotide with an anti-BrdU antibody .
Contents
BrdU Labeling Reagent, 1000x
Washing Buffer concentrate, 10x
Incubation Buffer
Nucleases
Anti-BrdU-POD, Fab fragments
Substrate Buffer
ABTS Substrate
Substrate Enhancer |