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Contents
BrdU Labeling Reagent, 1,000x conc.
Washing Buffer concentrate, 10x
Incubation Buffer
Anti-BrdU, containing nucleases for DNA denaturation
Anti-mouse Ig-alkaline Phosphatase
NBT
BCIP
Background Information
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3 H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2'-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Product Description
Sample material: Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).
Specificity: Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2'-deoxy-uridine, and shows crossreactivity with 5-iodo-2'-deoxy-uridine (10%). Anti-BrdU shows no crossreactivity with 5'-fluoro-2'-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.
Benefits
Safe: No radioisotopes are used
Easy to perform: Follows a standard immunohistochemistry protocol
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU
Flexible: Allows double-labeling protocols
Application
The kit is used for the detection of BrdU incorporated into cellular DNA by immunocyto/histochemistry.
5-Bromo-2'-deoxy-uridine Labeling and Detection Kit II
11299964001 1 kit (for up to 100 tests) |