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SuperScript® II Reverse Transcriptase
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.
• Yield – Reduced RNase H activity results in greater first-strand cDNA yields
• Size of cDNA – Generation of RT-PCR products up to 12 kb
• Thermostability – Full activity at 42°C
• Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® II is the amount of enzyme
Optimal Reaction Temperature: 42° C
Final Product Size(s): 12.3 kb or less
Reverse Transcriptase: SuperScript® II
Ribonuclease H Activity: Reduced
Form: Frozen
Format: Tube(s)
Quantity: 2000 units
Template: RNA-DNA Hybrid,
ssRNA,
ssDNA
Sensitivity: Medium
Product Size: 2,000 units
Concentration: 200 U⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Wet Ice
Number of Reactions: 10 Reactions
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. |