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原装现货|SUPERSCRIPT II
描述:
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.
Yield – Reduced RNase H activity results in greater first-strand cDNA yields
Size of cDNA – Generation of RT-PCR products up to 12 kb
Thermostability – Full activity at 42°C
Flexibility – Uses either total or poly(A)+ RNA
详细说明:
Optimal Reaction Temperature: 42° C
Final Product Size(s): 12.3 kb or less
Ribonuclease H Activity: Reduced
Concentration: 200 units/µl
Form: Frozen
Format: Tube(s)
Template: ssRNA,
ssDNA,
RNA-DNA Hybrid
Sensitivity: Medium
Product Size: 10,000 units
Enzyme Function: RNA dependent DNA Polymerase
Number of Reactions: 50 Reactions
内容和储存:
SuperScript® II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored. |