Uracil DNA Glycosylase
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Enzyme: UDG
Product Size: 100 units
Shipping Condition: Approved for shipment on Wet or Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Store in freezer (-5 to -30°C). |