E. COLI DNA LIGASE

E. coli DNA Ligase
E. coli DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of β-NAD between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate cohesive termini. Single-stranded nucleic acids are not substrates for this enzyme.
Applications: Second-strand cDNA synthesis (1). T4 DNA Ligase alternative when blunt-end ligation is not required (2).
Source: Purified from E. coli 594 (Su- ) bearing λ lysogen gt4lop-11 lig+ S7 (3).
Performance and Quality Testing: Endodeoxyribonuclease and 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested.
Unit Definition: One unit is the amount of enzyme required to give 50% ligation of Hind III-digested λ DNA in 30 min. at 16°C in a final volume of 20 µl containing a 5´ termini concentration of 0.12 ´M (300 µg/ml).
Enzyme: Ligase 
Product Size: 100 units 
Shipping Condition: Approved for shipment on Wet or Dry Ice 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.