Gateway® pET-DEST42 Vector The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers: ■Bacteriophage T7lac promoter for high-level expression O operator sequences for lac repressor binding to ensure tighter regulation of transcription ■pBR322 ori for minimal basal expression ■C-terminal V5 and 6xHis tags for efficient detection and purification Vector Type: pDEST, pET Cloning Method: Gateway® Promoter: T7 and lacO Protein Tag or Fusion: V5 Epitope Tag, His Tag (6x) Antibiotic Resistance (Bacterial): Ampicillin (AmpR) Product Size: 6 µg Inducing Agent: IPTG Selection Agent (Eukaryotic): None Constitutive or Inducible System: Inducible Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. The Champion™ pET-DEST42 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.