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Gateway® pcDNA™-DEST40 Vector
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway® pcDNA™-DEST40 vector offers the following key features:
•C-terminal V5-6x His tag for easy detection and rapid purification
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.
Promoter: CMV
Key Function: Constitutive Expression
Protein Tag or Fusion: His Tag (6x),
V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector Type: pDEST
Product Size: 6 µg
Constitutive or Inducible System: Constitutive
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
All destination vectors are provided lyophilized and supercoiled. |