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Dynabeads® Untouched™ Human B Cells Kit
Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.
■High purity, recovery and viability of human untouched B cells
■Easy to use and to scale up
■No columns required
An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.
Form: Bead
Capacity: Processes ~1x10^9 cells⁄kit
Cell Type: B Cells
Product Size: 1 kit
Final Product: Cells
Target Species: Human
Isolation Method: Negative Isolation
Output Viability: >97%
Number of Reactions: 20 Reactions
Sample Type (Specific): MNC
Starting Material (Cell #): ~5x10^7 cells/test
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
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