MAX Efficiency® Stbl2™ Competent Cells
MAX Efficiency® Stbl2™ 感受态细胞是专门为克隆不稳定插入片段设计的高效化学感受态细胞。 除 recA1 外,一套独特的遗传标记物确保正向重复序列和逆转录病毒序列 (1) 及串联阵列基因 (2) 能得到稳定克隆。 MAX Efficiency® Stbl2™ 感受态细胞提供:
■>1 x 109 转化子/flg,以克隆稀有序列
■消除 mcrBC-hsdRMS-mrr 和 mcrA 的突变,以克隆甲基化基因组序列 (1)
■独特的基因型稳定了正向重复和逆转录病毒序列,例如 SIV 和 HIV (1)
■末端A1 突变极大地增加了质粒产量和品质
Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: Stbl2™
Plasmid Type: May be used for plasmids > 20 kb
Product Size: 10 reactions
Blue-White Screening: No
Cloning Unstable DNA: Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Contains:
• MAX Efficiency® Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |