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Quantitative Pseudopodia Assay Kit
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PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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Description:
Quantitative Pseudopodia Assay Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Chemotaxis, or directed cell movement is a fundamental cellular process, necessary for embryonic development, angiogenesis, wound healing, and immune responses, as well as pathological events associated with inflammation and cancer metastasis [Lauffenburger & Horwitz, 1996]. Prior to cell body translocation or chemotaxis, a cell must first extend a dominant pseudopodium (lamellipodium) in the direction of the chemoattractant gradient [Lauffenburger & Horwitz, 1996; Parent & Devreotes, 1999]. Once a dominant pseudopodium is formed, cell movement can begin in the direction of the gradient. Formation of a directional pseudopodium is tightly controlled by signal transduction mechanisms that temporally and spatially regulate changes in focal adhesions, microtubules, and the actin/myosin cytoskeleton [Ridley et al., 1992; Schmidt et al., 1993; Manes et al., 1999; Nobes & Hall, 1999; Eddy et al., 2000].
The CHEMICON Quantitative Pseudopodia Assay Kit is a novel system designed to specifically isolate and quantitate extending or retracting pseudopodia from the cell body [Cho & Klemke, 2002]. This assay provides a quick and efficient system to aid in measuring the effects of various natural or synthetic compounds that specifically impact the microenvironment of the pseudopodial organelle including signaling processes, integrins or other adhesion receptors, and unique gene products. The assay is ideal for screening pharmacological agents that either stimulate or inhibit pseudopodia formation as well as for the identification of novel drug targets that impact cell chemotaxis.
In addition, Chemicon also provides QCM™ 24-well insert cell migration assay systems, CytoMatrix™ Cell Adhesion strips coated with ECM proteins or anti integrin antibodies, QCM Chemotaxis 96-well cell migration assay, and QuantiMatrix™ ECM protein ELISA kits
For Research Use Only; Not for use in diagnostic procedures
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Applications:
The Quantitative Pseudopodia Assay Kit is a novel system designed to specifically isolate & quantitate extending or retracting pseudopodia from the cell body.
Key Applications:
Activity Assay
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Components:
Pseudopodia Quantification Inserts - (Part No. 71611) - 1 X 24 well plate containing 12 6.5 micrometer transwells.
Cottons Swabs - (Part No. 10202) - 14 swabs
Pseudopodia Stain Solution - (Part No. 71605) 20 mL
Forceps - (Part No.10203) 1 pair of forceps
Pseudopodia Stain Extraction Buffer - (Part No. 71604) 20 mL
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Brand Family:
Chemicon
Storage Conditions:
Store Kit and Materials at 2-8ºC until their expiration date
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