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Endothelial Cell Adhesion Assay Kit
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PRODUCT FAMILY INFORMATION
Cell Based Assays
Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell, and cell-based activity assays. Study Apoptosis, Angiogensis, Adhesion and more.
EMD Millipore offers a significant portfolio of well-published, quantitative and optimized live cell, whole-cell and cell-based activity assays that mimic native environments for direct and indirect detection of cellular response.
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Description:
Endothelial Cell Adhesion Assay Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Introduction:
Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation and angiogenesis. The recruitment of leukocytes into inflammatory tissues is regulated by the interaction between blood cells and endothelial cells that is preceded by integrin-mediated cell adhesion. Endothelial-leukocyte cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Atherosclerosis, ulcer, myocardial infarction, stroke, and other inflammatory processes are all related to, and dependent on this endothelial-leukocyte adhesion process.
Expression of surface molecules on the vascular endothelium is altered at sites of pathological inflammation. Endothelial Cell Adhesion Molecules (ECAMs) are important mediators of leukocyte recruitment and adherence to the endothelium. ECAMs such as E-selectin, VCAM-1, and ICAM-1 are upregulated during inflammation, which initiates leukocyte adhesion to the endothelium, and ultimately contributes to disease progression or tissue damage. Expression of these ECAMs is mediated by proinflammatory cytokines such as Interleukin-1 beta and Tumor Necrosis Factor alpha, which propel the interaction of integrin multimers on the leukocyte cell surface and the ECAMs on the endothelial cells. The genes encoding ECAMs contain overlapping control mechanisms that allow a single signaling pathway to upregulate several genes with the potential to change the endothelial phenotype. Since ECAMs are regulated at the gene level by transcription factors, proteasome inhibitors can modulate cytokine-induced expression of ECAMs and subsequent leukocyte adhesion to the endothelium. Controlling and manipulating the endothelial-leukoctye adhesion process will create new avenues for drug therapies for vascular and inflammatory disease states.
The CHEMICON® Endothelial Cell Adhesion Assay kit (ECM645) allows the researcher to test a variety of cell types that interact with activated or inactivated endothelial cell layers. Human umbilical vein endothelial cells (HUVECs) from ATCC® (CRL-1730) or Cambrex© (CC-2519) are ideal for measuring endothelial adhesion activity with the assay. The CHEMICON® Endothelial Cell Adhesion Assay kit includes a 96 well tissue culture treated fluorescence microtiter plate and reagents that allow for large-scale screening and quantitative comparison of multiple samples/cell line adhesion to an endothelial monolayer. Once endothelial cells are seeded in the tissue culture plate, they can be treated with cytokines, chemokines, transcription regulators, or selective adhesion inhibitors. The protein synthesis inhibitor Cycloheximide and the RNA synthesis inhibitor Actinomycin D are provided as controls for adhesion protein expression studies. Treatment of endothelial cells, such as human umbilical vein endothelial cells (HUVECs), with pro-inflammatory cytokines such as Interleukin-1ß eta or Tumor Necrosis Factor-a can induce expression of ECAMs, as well as othe |