|
ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set
Close
REFERENCES
The Inducible Tissue-Specific Expression of the Human IL-3/GM-CSF Locus Is Controlled by a Complex Array of Developmentally Regulated Enhancers.
Euan W Baxter,Fabio Mirabella,Sarion R Bowers,Sally R James,Aude-Marine Bonavita,Elisabeth Bertrand,Ruslan Strogantsev,Abbas Hawwari,Andrew G Bert,Andrea Gonzalez de Arce,Adam G West,Constanze Bonifer,Peter N Cockerill (2012) Journal of immunology (Baltimore, Md. : 1950).189
Chromatin modifications induced by the AML1-ETO fusion protein reversibly silence its genomic targets through AML1 and Sp1 binding motifs.
A Maiques-Diaz,F S Chou,M Wunderlich,G Gómez-López,F V Jacinto,S Rodriguez-Perales,M J Larrayoz,M J Calasanz,J C Mulloy,J C Cigudosa,S Alvarez (2012) Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K.26
Combined chromatin and expression analysis reveals specific regulatory mechanisms within cytokine genes in the macrophage early immune response.
Maria Jesus Iglesias,Sarah-Jayne Reilly,Olof Emanuelsson,Bengt Sennblad,Mohammad Pirmoradian Najafabadi,Lasse Folkersen,Anders Mälarstig,Jens Lagergren,Per Eriksson,Anders Hamsten,Jacob Odeberg (2012) PloS one.7
Identification and characterization of the human NOL7 gene promoter.
Tanmayi P Mankame,Guolin Zhou,Mark W Lingen (2010) Gene.456
View All
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ChIP Rabbit Purified Polyclonal Antibody
Description:
ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin.
The qPCR primers included flank the Sp1 binding site in human DHFR promoter.
View All »
Specificity:
Sp1
Molecular Weight:
105 kDa and 95 kDa
Immunogen:
Full length human Sp1 protein
Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western Blot Analysis:
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Control:
Includes negative control rabbit IgG and control primers specific for human dihydrofolate reductase (DHFR) promoter.
Quality Assurance:
Chromatin immunoprecipitation:
Sonicated chromatin prepared from 1 x 106 HeLa cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPTM A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR flanking the human DHFR promoter that contains a Sp1 binding site (Please see figures).
Please refer to the EZ-Magna ChIP™ A (Cat.# 17-408)
or EZ-ChIP™ (Cat.# 17-371) kit protocols for
experimental details.
View All »
Purification Method:
Protein A purfied
Presentation:
Anti-Sp1 (Rabbit polyclonal IgG). 1 vial containing 100 μg of protein A purified IgG in 100 μL of 0.014 M |