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ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal
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REFERENCES
Defining the CREB regulon: a genome-wide analysis of transcription factor regulatory regions.
Impey, Soren, et al. (2004) Cell, 119: 1041-54 (2004)
Genome-wide analysis of CREB target genes reveals a core promoter requirement for cAMP responsiveness.
Conkright, Michael D, et al. (2003) Mol. Cell, 11: 1101-8 (2003)
The induction of cyclooxygenase-2 mRNA in macrophages is biphasic and requires both CCAAT enhancer-binding protein beta (C/EBP beta ) and C/EBP delta transcription factors.
Caivano, M, et al. (2001) J. Biol. Chem., 276: 48693-701 (2001)
Follicle-stimulating hormone stimulates protein kinase A-mediated histone H3 phosphorylation and acetylation leading to select gene activation in ovarian granulosa cells.
Salvador, L M, et al. (2001) J. Biol. Chem., 276: 40146-55 (2001)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ChIP Rabbit Culture Supernatant Monoclonal Antibody
Description:
ChIPAb+ CREB - ChIP Validated Antibody and Primer Set, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. CREB antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the CREB antibody is functionally validated in the precipitation of CREB associated chromatin.
The qPCR primers included flank the CREB binding site in human NR4A2 promoter.
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Specificity:
Recognizes CREB, MW ~43 kDa.
Molecular Weight:
~43 kDa
Epitope:
a.a. 5-24
Immunogen:
CREB purified antibody is made against a KLH-conjugated synthetic peptide corre-sponding to amino acids 5-24 of human CREB.
Isotype:
IgG
Background Information:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ CREB, antibody/primer set includes the CREB, antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 64 bp region flanking the CRE of the human cFos gene. The CREB and negative control cultured supernatant are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CREB associated chromatin.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse, rat. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from 293T cells (5 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 10 µL of either a negative supernatant, or CREB antibody and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of CREB-associated DNA fragments was verified by qPCR using cFos downstream 4Kb (negative) and cF |