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Anti-gp91-phox Antibody
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REFERENCES
Angiotensin Receptor Mediated Oxidative Stress Is Associated with Impaired Cardiac Redox Signaling and Mitochondrial Function in Insulin Resistant Rats.
Vazquez-Medina, Jose Pablo, et al. (2013) Am. J. Physiol. Heart Circ. Physiol., (2013)
Endothelial function and vascular oxidative stress in long-lived GH/IGF-deficient Ames dwarf mice.
Anna Csiszar,Nazar Labinskyy,Viviana Perez,Fabio A Recchia,Andrej Podlutsky,Partha Mukhopadhyay,Gyorgy Losonczy,Pal Pacher,Steven N Austad,Andrzej Bartke,Zoltan Ungvari (2008) American journal of physiology. Heart and circulatory physiology.295
Redox survival signalling in retina-derived 661W cells.
A M Mackey,N Sanvicens,G Groeger,F Doonan,D Wallace,T G Cotter (2008) Cell death and differentiation.15
Vascular aging in the longest-living rodent, the naked mole rat.
Anna Csiszar, Nazar Labinskyy, Zsuzsanna Orosz, Zhao Xiangmin, Rochelle Buffenstein, Zoltan Ungvari (2007) American journal of physiology. Heart and circulatory physiology.293:H919-27
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Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Purified Polyclonal Antibody
Description:
Anti-gp91-phox Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Detects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.
Molecular Weight:
Varies. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr ~75 kDa, or appear as a smear between 75-91 kDa.
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.
Isotype:
IgG
Background Information:
Cytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein.
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Predicted to cross react with mouse.
Application Notes:
Immunoprecipitation:
Not recommended.
Control:
Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on human neutrophil membranes.
Western Blot Analysis:
1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.
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Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the v |