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Anti-Mn-SOD Antibody
Species Reactivity Key Applications Host Format Antibody Type
H, R, M, B, not Ca IHC, IH(P), WB Rabbit Purified Polyclonal Antibody
Description:
Anti-Mn-SOD Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes MnSOD, Mr 24 kDa.
Molecular Weight:
~24 kDa
Immunogen:
Recombinant human MnSOD (manganous superoxide dismutase).
Isotype:
IgG
Background Information:
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.
Species Reactivity:
Human
Rat
Mouse
Bovine
Species Reactivity Note:
Human, mouse, rat and bovine. Not canine (dog).
Non-Reactive Species:
Canine
Application Notes:
Immunohistochemistry (Paraffin):
10 μg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections.
Control:
L6 cell lysate, rat brain tissue extract.
Quality Assurance:
Evaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 μg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at 2 to 8ºC from date of receipt.
UniProt Number:
P04179
Entrez Gene Number:
NP_000627
Gene Symbol:
SOD2
Mn-SOD
MNSOD
MnSOD
IPO-B
Alternate Names:
Mn superoxide dismutase
indophenoloxidase B
manganese superoxide dismutase
manganese-containing superoxide dismutase
mangano-superoxide dismutase
superoxide dismutase 2, mitochondrial
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunohistochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Entrez Gene Summary:
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UniProt Su |